протокол практического занятия 2 (частная микробиология). Газовая гангрена
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ВОЗБУДИТЕЛИ РАНЕВЫХ АНАЭРОБНЫХ ИНФЕКЦИЙ: АНАЭРОБНАЯ ГАЗОВАЯ ИНФЕКЦИЯ, СТОЛБНЯК, НЕКЛОСТРИДИАЛЬНЫЕ АНАЭРОБНЫЕ ИНФЕКЦИИ. ГАЗОВАЯ ГАНГРЕНА Семейство_______________Род_______________Виды____________________________ __________________________________________________________________________________________________________________________________________________________ Морфологические свойства. _________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Cl. perfringens. Окраска по Граму Культуральные свойства. _________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Рост Cl. perfringens на: среде Вильсона-Блер среде Цейсслера   Токсинообразование _________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Факторы вирулентности Cl. perfringens:
Антигенная структура: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Источник инфекции - ___________________________________________________ Механизм передачи - ___________________________________________________ Факторы, способствующие развитию заболевания: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Механизм действия экзотоксина Cl. perfringens - _________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Основные клинические проявления: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________Препараты для специфической терапии: Противогангренозная сыворотка - ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Препараты для специфической профилактики: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________. ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ СТОЛБНЯК Семейство_______________Род_______________Вид____________________________ _____________________________________________________________________________ Морфологические свойства. ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Cl. tetani Окраска по Граму Биохимические свойства: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Факторы вирулентности Cl. tetani: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Антигенная структура: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Источник инфекции - ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Механизм передачи - _________________________________________________________ Факторы, способствующие развитию заболевания: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Механизм действия экзотоксина Cl.tetani - ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Основные клинические проявления: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Препараты для специфической терапии: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________Препараты для специфической профилактики: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Экстренная профилактика: а) столбнячный анатоксин; б) противостолбнячная сыворотка. Микробиологическая диагностика столбняка ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Микроскопический метод ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Бактериологический метод. ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ _______________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ЛАБОРАТОРНАЯ ДИАГНОСТИКА АНАЭРОБНЫХ ИНФЕКЦИЙ. 1 Бактериологический метод. Исследуемый материал - __________________________________________________ Метод окраски препарата - ______________________________________________ 2. Бактериологический метод. Исследуемый материал - ________________________________________________________ 1 этап. Обогащение. Посев исследуемого материала на__________________________________________ 2 этап. Выделение чистой культуры Высев со среды______________________ на среду _______________ Инкубация посевов в _______________________ 3 этап. Изучение культуральных, морфологических свойств. Учет культуральных свойств на среде ____________________________ Микроскопия мазка по _______________________________________ Откол колоний на ____________________________________________ 4 этап. Изучение свойств, необходимых для идентификации культуры (биохимические свойства; токсигенность). 5 этап. Идентификация выделенной культуры. ВОЗБУДИТЕЛИ АНАЭРОБНОЙ (НЕКЛОСТРИДИАЛЬНОЙ) ИНФЕКЦИИ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Семейство_______________Род_______________Вид____________________________ _____________________________________________________________________________ Род Bacteroides ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Род Porphyromonas ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Род Prevotella ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Род Fusobacterium ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ЛАБОРАТОРНАЯ ДИАГНОСТИКА ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ |